Kinetic and spectral properties of pea cytosolic ascorbate peroxidase
نویسندگان
چکیده
منابع مشابه
Characterization and crystallization of recombinant pea cytosolic ascorbate peroxidase.
An Escherichia coli expression system has been developed for pea cytosolic ascorbate peroxidase (APX). The enzyme was expressed as a fusion product with the E. coli maltose-binding protein for rapid, affinity chromatography purification. Recombinant ascorbate peroxidase (rAPX) was purified by tryptic digestion to separate the maltose-binding protein from rAPX followed by three chromatographic s...
متن کاملAbolishing activity against ascorbate in a cytosolic ascorbate peroxidase from switchgrass.
Switchgrass (Panicum virgatum L.) is being developed as a bioenergy species. Recently an early version of its genome has been released permitting a route to the cloning and analysis of key proteins. Ascorbate peroxidases (APx) are an important part of the antioxidant defense system of plant cells and present a well studied model to understand structure-function relationships. Analysis of the ge...
متن کاملInvolvement of cytosolic ascorbate peroxidase and Cu/Zn-superoxide dismutase for improved tolerance against drought stress.
In order to understand the role of cytosolic antioxidant enzymes in drought stress protection, transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants overexpressing cytosolic Cu/Zn-superoxide dismutase (cytsod) (EC 1.15.1.1) or ascorbate peroxidase (cytapx) (EC 1.11.1.1) alone, or in combination, were produced and tested for tolerance against mild water stress. The results showed that the sim...
متن کاملDual regulation of cytosolic ascorbate peroxidase (APX) by tyrosine nitration and S-nitrosylation
Post-translational modifications (PTMs) mediated by nitric oxide (NO)-derived molecules have become a new area of research, as they can modulate the function of target proteins. Proteomic data have shown that ascorbate peroxidase (APX) is one of the potential targets of PTMs mediated by NO-derived molecules. Using recombinant pea cytosolic APX, the impact of peroxynitrite (ONOO-) and S-nitrosog...
متن کاملIsolation and characterization of a cDNA for spinach cytosolic ascorbate peroxidase.
Reduced oxygen intermediates are produced in plant tissues from mitochondrial and chloroplastic electron transport pathways and from electron-saturated photosynthetic pigments (Bowler et al., 1992). For instance, the Mehler reaction is a primary source of reduced oxygen intermediates in chloroplasts in which dioxygen is reduced by Fd on the reducing side of PSI to produce .O2-. The dismutation ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1996
ISSN: 0014-5793
DOI: 10.1016/0014-5793(96)00562-5